Mark A. Rutherford, PhD

Assistant Professor of Otolaryngology

Rutherford Lab Website

Mailing Address:

Washington University School of Medicine
Department of Otolaryngology-Head and Neck Surgery
660 S. Euclid Ave.-Campus Box 8115
St. Louis, MO 63110

Electrical and chemical signaling across membranes, neural temporal code, spike generation, molecular anatomy of synapses, membrane excitability disorders, auditory and vestibular systems, synaptic electrophysiology, sensory hair cells, spiral ganglion neurons, ribbon synapses, ion channel organization and function, nano-scale microscopy, Ca2+ and voltage imaging, mutational analysis, computational modeling, cochlear implants, structure-function relationships, synaptic development and heterogeneity, genetics of hearing and deafness, optogenetics, mitochondria, network and synaptic plasticity, efferent neuromodulation of sensory processing, prevention of hearing loss.


Education

  • 2009-2012 Postdoctoral Fellow, University of Göttingen
  • 2005-2009 Postdoctoral Training, University of Oregon
  • 2000-2005 Ph.D., Biology, University of Oregon
  • 1995-1999 B.S., Nutritional Sciences, University of Missouri

Honors

  • 2009-2012 Research Fellow, Alexander von Humboldt Foundation Postdoctoral Grant
  • 2002 Scholarship, Neural Systems and Behavior, Marine Biological Laboratory, Woods Hole, Massachusetts
  • 2001 Development Training Grant, Pre-synaptic Mechanisms, Inst. of Neurosci., University of Oregon
  • 1999 Graduation with Honors, University of Missouri
  • 1995-1999 National Merit Scholar, University of Missouri

Selected Publications

  1. Lars Becker, Michael E. Schnee, Mamiko Niwa, Willy Sun, Stephan Maxeiner, Sara Talaei, Bechara Kachar, Mark A. Rutherford, Anthony J. Ricci. The presynaptic ribbon maintains vesicle populations at the hair cell afferent fiber synapse. eLife, 7, e30241 (2018). PMID: 29328021
  2. Sebe, J.Y., Cho, S., Sheets, L., Rutherford, M.A., von Gersdorff, H., and Raible, D.W.  Ca2+-Permeable AMPARs Mediate Glutamatergic Transmission and Excitotoxic Damage at the Hair Cell Ribbon Synapse. J Neurosci  37.25 (2017): 6162-6175. PMID: 28539424
  3. Hirose, K., Rutherford, M.A., and Warchol, M.E. Two cell populations participate in clearance of damaged hair cells from the sensory epithelia of the inner ear. Hearing Research 352: 70 (2017). PMID: 28526177
  4. Ohn, T.L., Rutherford, M.A., Jing, Z., Jung, S., Duque-Afonso, C.J.,Hoch, G., Picher, M.M., Scharinger, A., Strenzke, N., and Moser, T.  Hair Cells Use Active Zones with Different Voltage Dependence of Ca2+ Influx to Decompose Sounds into Complementary Neural Codes. PNAS 113(32): E4716 (2016) PMID: 27462107
  5. Kim, K.X., Rutherford, M.A. Maturation of NaV and KV Channel Topographies in the Auditory Nerve Spike Initiator before and after Developmental Onset of Hearing Function. J Neurosci 36(7): 2111 (2016) PMID: 26888923 
  6. Rutherford, M.A. Resolving the Structure of Inner Ear Ribbon Synapses with STED Microscopy. Synapse 69(5):242 (2015) PMID: 25682928 Wong, A.B., Rutherford, M.A., Gabrielaitis, M., Pangršič, T., Göttfert, F., Frank, T., Michanski, F., Hell, S., Wolf, F., Wichman, C., Moser, T.  Developmental Refinement of Hair Cell Synapses Tightens the Coupling of Ca2+ Influx to Exocytosis. EMBO J 33(3):247 (2014) PMID: 24442635  
  7. Jing, Z., Rutherford, M.A., Takago, H., Frank, T., Fejtova, A., Khimich, D., Moser, T., Strenzke, N.  Disruption of the Presynaptic Cytomatrix Protein Bassoon Degrades Ribbon Anchorage, Multi-quantal Release, and Sound Encoding at the Hair Cell Afferent Synapse. J Neurosci 33(10):4456 (2013) PMID: 23467361
  8. von Ameln, S., Wang, G., Boulouiz, R., Rutherford, M.A., Smith, G.M., Li, Y., Pogoda, H.M., Nürnberg, G., Volk, A.E., Stiller, B., Hong, J.S., Goodyear, R.J., Nürnberg, P., Richardson, G.P., Hammerschmidt, M., Moser, T., Wollnik, B., Koehler, C.M., Teitell, M.A., Barakat, A., Kubisch, C.  A Mutation in PNPT1, Encoding Mitochondrial-RNA-Import Protein PNPase, Causes Hereditary Hearing Loss. The American Journal of Human Genetics 91(5):919 (2012) PMID: 23084290
  9. Rutherford, M.A., Pangršič, T.  Molecular Anatomy and Physiology of Exocytosis in Sensory Hair Cells. Cell Calcium 52(3-4):327 (2012) PMID: 22682011 Rutherford, M.A., Chapochnikov, N.M., Moser, T.  Spike Encoding of Neurotransmitter Release Timing by Spiral Ganglion Neurons of the Cochlea. J Neurosci 32(14):4773 (2012) PMID: 22492033
  10. Frank, T., Rutherford, M.A., Strenzke, N., Neef, A., Pangršič, T., Khimich, D., Fetjova, A., Gundelfinger, E.D., Liberman, M.C., Harke, B., Bryan, K.E., Lee, A., Egner, A., Riedel, D., Moser, T.  Bassoon and the Synaptic Ribbon Organize Ca2+ Channels and Vesicles to Add Release Sites and Promote Refilling. Neuron 68(4):724 (2010) PMID: 2109286